Cell dating

Current atmospheric C14 is about twice the level it was before the 1950s.First author Kristy Spalding and colleagues capitalized on this relatively rapid decline in C14 to develop their dating method.Indeed, when Spalding and colleagues examined samples that dated prior to or after the proliferation of atmospheric nuclear tests, they found that the C14 content in the DNA correlated with the predicted atmospheric C14 at the time the DNA would have been synthesized.Next, examining samples from single individuals born after the test ban treaty went into effect, the authors found that the C14 content in DNA isolated from the cerebellum, cortex and intestine were, as would be expected, the same age.Cerebellar DNA was a few years older than the DNA in the cortex, which in turn was about 12 years older than the DNA in the small intestine.This matches the pattern that would be expected when one considers the time taken for development of the human brain and the relatively rapid turnover of epithelial cells in the intestine.While they found that total DNA C14 levels in these samples are younger than the donor, indicating cell turnover, they found that the cortical neurons are always as old as the individual donor.This was true for people born prior to, during, or after the spike in atmospheric C14.

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To tackle this question, the authors isolated neuronal and non-neuronal nuclei from cerebral cortex tissue samples.Start meeting singles in Mobile today with our free online personals and free Mobile chat!Mobile is full of single men and women like you looking for dates, lovers, friendship, and fun.The authors estimated that using their method, they can put an age on human cells to within /- 2 years.Turning to individual cell types rather than tissues, Spalding and colleagues set about to determine if cortical neurons are as old, or perhaps younger, than the individual.

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